报告题目 | Flow Field-Flow Fractionation and Mass Spectrometry for Bio-macromolecules |
报告人 | Prof. Myeong Hee Moon |
报告人单位 | Yonsei University, Republic of Korea |
报告时间 | 2024年12月10日（星期二）16：00 |
报告地点 | 理化大楼3016会议室 |
主办单位 | 精准智能化学重点实验室 |
报告摘要 | Flow field-flow fractionation (FlFFF) is an elution-based separation method capable of fractionating particles or macromolecules by size. Separation in FlFFF is carried out in a thin empty channel space using two different flow streams, migration flow and crossflow. Due to the open channel structure without packing materials, FlFFF is suitable for separating biomacromolecules and bioparticles without being denatured or dissociated owing to the use of aqueous carrier liquids. This presentation will show the analytical approaches for size-dependent lipidomic analysis of bio-nanoparticles by using an offline and online combination of FlFFF with mass spectrometry (MS). FlFFF was utilized to separate lipoproteins by sizes from blood plasma of patients with coronary artery disease and Alzheimer’s disease, exosomes from patients’ urine with prostate cancer, exosomes and microvesicles secreted from cells, followed by the lipidomic analysis of collected fractions using nanoflow UHPLC-ESI-MS/MS. FlFFF can be directly coupled with ESI-MS for top-down lipidomic analysis by using a miniaturized FlFFF (mAF4) channel and mAF4-ESI-MS/MS was applied to hepatocellular carcinoma (HCC) plasma samples for targeted quantification of 25 lipid biomarker candidates. This demonstrates the potential of mAF4-ESI-MS/MS for the high-speed screening of targeted lipid species in specific lipoprotein components of blood without lipid extraction. |
报告人简介 | Prof. Myeong Hee Moon completed his undergraduate studies in Chemistry at Yonsei University in 1987 and received his Ph.D. in Chemistry from the University of Utah in 1991 under Prof. Calvin J. Giddings. He began his academic career at Kangnung National University in 1994 and joined Yonsei University's Chemistry Department in 2003. His research focuses on developing methodologies in flow field-flow fractionation (FFF) for separating and characterizing biological macromolecules. He has developed various FFF channel systems, including frit-inlet asymmetrical flow FFF, hollow fiber flow FFF (HF-FlFFF), multidimensional separation techniques using isoelectric focusing and asymmetrical FlFFF (IEF-AF4) for protein separation, and miniaturized AF4 for direct lipid analysis with ESI-MS/MS. He has published over 200 scientific articles. He served as president of the Korean Society of Mass Spectrometry, vice-president of the Korean Chemical Society, Executive Committee Member of the International Mass Spectrometry Foundation, and chair of the SBFFF of the International Symposium on Field and Flow-Based Separation. |
